《植物生理学报》 2011, 47(10): 987-990

南荻的组织培养与快速繁殖技术

郭夏宇, 李合松, 彭克勤, 黄志刚, 萧浪涛*

湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙410128

通信作者：萧浪涛；E-mail: langtaoxiao@163.com；Tel: 0731-84635261

摘　要：

以南荻幼穗为外植体, 进行了组织培养与快速繁殖技术的研究。结果表明: 最佳诱导培养基: MS+2.0 mg•L-1 2,4- D+0.1 mg•L-1 6-BA+0.5 g•L-1水解酪蛋白+0.5 g•L-1脯氨酸; 最佳分化培养基: MS+0.5 mg•L-1 NAA+0.5 mg•L-1 KT+0.5 mg•L-1 IAA+2.0 mg•L-1 6-BA+0.5 g•L-1水解酪蛋白+0.5 g•L-1脯氨酸; 最佳生根培养基: MS+1.0 mg•L-1 NAA+0.25 mg•L-1 Met。炼苗 后, 移入营养土与珍珠岩(1:1)的基质中, 移栽成活率高达100%。该体系的建立为南荻规模化生产及遗传改良提供了前提条件。

关键词：南荻; 组织培养; 快速繁殖

收稿：2011-07-28   修定：2011-08-25

资助：国家“973”前期研究专项(2010CB134403)和湖南省科技计划项目(2009FJ1004-2、2009WK4002和2011FJ3015)。

Tissue Culture and Rapid Propagation Technology of Triarrhena lutarioriparia L. Liu sp. nov.

GUO Xia-Yu, LI He-Song, PENG Ke-Qin, HUANG Zhi-Gang, XIAO Lang-Tao*

Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China

Corresponding author: XIAO Lang-Tao; E-mail: langtaoxiao@163.com; Tel: 0731-84635261

Abstract:

Young ears of Triarrhena lutarioriparia were used as explant in the study of tissue culture and rapid propagation. The results indicate that the best induced medium for callus induction was MS medium with 2.0 mg·L^-1 2,4-D, 1.0 mg·L^-1 6-BA ,0.5 g·L^-1 casein acids hydrolysate and 0.5 g·L^-1 proline. The best medium for bud differentiation was MS medium with 0.5 mg·L^-1 NAA, 0.5 mg·L^-1 KT, 0.5 mg·L^-1 IAA, 2.0 mg·L^-1 6-BA, 0.5 g·L^-1casein acids hydrolysate and 0.5 g·L^-1 proline. The best medium for root differentiation was MS medium with 1.0 mg·L^-1 NAA and 0.25 mg·L^-1Met. After acclimatization, the culture-bottle seedlings were transplanted into base materials which contains nutritious soil and perlite (1:1). The survival rate was up to 100%. The established system provided preconditions for large-scale production and genetic modification improvement of Triarrhena lutarioriparia.

Key words: Triarrhena lutarioriparia L. Liou. sp. nov; tissue culture; rapid propagation